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- Title
Changes in Angiotensin II Type 1 Receptor Expression in the Rat Bladder by Bladder Outlet Obstruction.
- Authors
ShoheiTobu; Noguchi, Mitsuru; Hatada, Teppei; Mori, Ken-ichi; Matsuo, Manabu; Sakai, Hideki
- Abstract
Purpose: To demonstrate the change in the expression of angiotensin II type 1 receptor (ATI) in the rat bladder with partial bladder outlet obstruction (P-BOO). Materials and Methods: Bladder specimens were obtained from 12-week-old Wistar female rats that were divided into two groups, a P-BOO group and a control group. The rats of the P-BOO group were divided into six groups: a sham-operated control group, 1 day postoperatively, 2 days postoperatively, 4 days postoperatively, 7 days postoperatively and 14 days postop-eratively. The cystometric findings and immunohistochemi-cal staining of the detrusor muscle with the ATI antibody were compared in each group. Results: AT1 localized on the cell membrane of the detrusor smooth muscle and in cyto-plasm of suburothelial myofibroblasts in the control rats. The expression of AT1 disappeared in the detrusor muscle and suburothelial myofibroblasts in P-BOO, but ATI was highly expressed in urothelial cells 1 day after surgery. The expression of AT1 in urothelial cells gradually decreased with time after surgery. AT1 completely disappeared in urothelial cells 14 days after surgery. Conclusions: The present study dem-onstrated that the site of AT1 expression changes in response to the mechanical stress caused by P-BOO, and finally there was no expression of AT1 in rat bladder tissue following P-BOO. These data suggest the change in AT1 expression may play a role in bladder function.
- Subjects
ANGIOTENSIN II; GENE expression; BLADDER diseases; IMMUNOHISTOCHEMISTRY; MYOFIBROBLASTS; CONTROL groups; LABORATORY rats
- Publication
Urologia Internationalis, 2012, Vol 89, Issue 2, p241
- ISSN
0042-1138
- Publication type
Article
- DOI
10.1159/000339373