We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.
- Authors
Lu, Chieh-Han; Tang, Wei-Chun; Liu, Yen-Ting; Chang, Shu-Wei; Wu, Frances Camille M.; Chen, Chin-Yi; Tsai, Yun-Chi; Yang, Shun-Min; Kuo, Chiung-Wen; Okada, Yasushi; Hwu, Yeu-Kuang; Chen, Peilin; Chen, Bi-Chang
- Abstract
Recent advances in super-resolution microscopy allow the localization of single molecules within individual cells but not within multiple whole cells due to weak signals from single molecules and slow acquisition process for point accumulation to reconstruct super-resolution images. Here, we report a fast, large-scale, and three-dimensional super-resolution fluorescence microscope based on single-wavelength Bessel lightsheet to selectively illuminate spontaneous blinking fluorophores tagged to the proteins of interest in space. Critical parameters such as labeling density, excitation power, and exposure time were systematically optimized resulting in a maximum imaging speed of 2.7 × 104 µm3 s−1. Fourier ring correlation analysis revealed a reconstructed image with a lateral resolution of ~75 nm through the accumulation of 250 image volumes on immobilized samples within 15 min. Hence, the designed system could open new insights into the discovery of complex biological structures and live 3D localization imaging. Lu, Tang, Liu et al. present a 3D super-resolution fluorescence microscope that uses selective plane illumination and a spontaneously blinking dye for localization-based imaging. They show that their microscope can be used to study complex biological structures.
- Subjects
CELL imaging; HIGH resolution imaging; FLUORESCENCE microscopy; IMMOBILIZED cells; CELL morphology
- Publication
Communications Biology, 2019, Vol 2, Issue 1, pN.PAG
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-019-0403-9