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- Title
Molecular Detection and Virulence Profiling of Associated Bacterial Pathogens Causing Bacterial Blight in Rice.
- Authors
REDDY, NAMBURI KARUNAKAR; KUMAR, M. K. PRASANNA; PATIL, SWATHI S.
- Abstract
Bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive diseases. The disease severity of this differs from geographical regions, strain (race) and the host genotype. Hence, isolates were collected from different locations of Karnataka to know the virulence of Xoo and recorded disease severity. Cultured isolates were cultured in the laboratory and were confirmed by 16S rRNA sequencing as well isolated Pantoea ananitis associated with bacterial blight and producing similar kind of symptoms as that of Xoo. The ClustalW analysis of the 16S rRNA gene sequences revealed two groups in the case of both Xoo and P. ananitis. The second group containing only two isolates Xoo 4 and Xoo 9 are more similar to the MH444307 isolate from Maharashtra and is delineated from the rest of the Xoo isolates. The 35 isolates of P. ananitis also formed into two groups and had more similarity with Japan isolate AB304809 followed by South Korean isolates HE672167 and HE716948 respectively. Around 42 resistance genes of rice (Xa genes), have been identified to confer resistance against various strains of Xoo. An understanding of pathotype diversity within the target pathogen population is required to identify the Xa genes to be deployed for developing resistant rice cultivars. The isolates of Xoo and P. ananitis collected from different parts of Karnataka formed 2 major pathotypes of both Xoo and P. ananitis and were distinguished based on their reaction towards Xa genes in the monogenic lines. The resistance gene Xa21 conferred resistance to more than 70 per cent of the Xoo and P. ananitis population. Further the amounts of exopolysaccharide (EPS) produced were quantified and varied depending on Xoo isolates. Isolate Xoo 3 produced a distinctly high amount of EPS (92.3 mg) followed by Xoo 6, Xoo 8 and Xoo 4. On the contrary, Xoo 2 produced distinctly less amount of EPS that has been co-related with the virulence nature of the pathogen on the rice cultivar, TN-1.
- Publication
Mysore Journal of Agricultural Sciences, 2023, Vol 57, Issue 2, p246
- ISSN
0047-8539
- Publication type
Article