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- Title
Neuroprotective and antioxidant activities of saponins' mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes.
- Authors
Kondeva-Burdina, Magdalena; Krasteva, Ilina; Popov, Georgi; Manov, Vasil
- Abstract
The aim of the study was to investigate the possible neuroprotective and antioxidant activity of purified saponins'mixture (PSM), isolated from Astragalus glycyphylloides (Fabaceae), in a model of 6-hydroxydipamine (6-OHDA)-induced oxidative stress on isolated rat brain synaptosomes. Synaptosomes were incubated with 3 different concentrations of PSM: 60 μg/mL; 6 μg/mL; 0.6 μg/ mL. The effects of PSM were compared to those of silymarin (S), at the same concentrations. The main parameters, characterized functional and metabolic status of synaptosomes, were investigated: viability (MTT-test) and level of reduced glutathione (GSH). At isolated rat brain synaptosomes, in conditions of 6-OHDA-induced oxidative stress (150 μM), PSM revealed statistically significant, concentration-dependent, neuroprotective and antioxidant effects, compared to those of silymarin. Effects were most prominent at concentration 60 μg/mL. These neuroprotective effects of PSM might be due to the possible activity as scavenger of reactive oxygen species (ROS), produced by p-quinone (toxic metabolite of 6-OHDA).
- Subjects
NEUROPROTECTIVE agents; ANTIOXIDANTS; SILYMARIN; OXIDATIVE stress; SYNAPTOSOMES
- Publication
Pharmacia (0428-0296), 2019, Vol 66, Issue 4, p233
- ISSN
0428-0296
- Publication type
Article
- DOI
10.3897/pharmacia.66.e37997