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- Title
DESINFESTAÇÃO DE REBENTOS DE SISAL PARA UTILIZAÇÃO IN VITRO.
- Authors
Sales Nobrega, Flavia Monique; da Cunha Soares, Taíza; Soares de Melo, Alberto; Frota Chagas Carvalho, Julita Maria
- Abstract
The sisal (Agave sisalana) is known worldwide for its high fiber content, which is widely used in the manufacture of utensils, as well as in the automotive industry, chemical industry, and construction. However, some factors may prevent the development of this culture, like diseases such as collar rot, caused by fungi of the genus Pythium sp. Trying to obtain healthy seedlings that are free from pathogens, this work set a protocol for efficient disinfection of sisal shoots, using different concentrations and different combinations of the antibiotic Citrofloxacino and the fungicide Baytan®. The explants were decontaminated with detergent solutions, sodium hypochlorite, and formaldehyde. This was followed by treatment with solutions containing different concentrations of the antibiotic and fungicide combinations. Then, they were cultured on MS medium. Evaluations were made at 7, 15, and 21 days after culture for analysis of the rate of bacterial and fungal contamination. Weobserved that the use of 0.5% Baytan® fungicide associated with 1% of the citrofloxacino antibiotic was the most effective in decontaminating explants sisal.
- Publication
Revista UniVap, 2015, Vol 21, Issue 37, p43
- ISSN
1517-3275
- Publication type
Article
- DOI
10.18066/revistaunivap.v21i37.291