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- Title
EXPLORING THE CORRELATION OF CATSPER SINGLE NUCLEOTIDE POLYMORPHISM (SNPS) AND GENE EXPRESSION OF ASTHENOZOOSPERMIC INFERTILE MEN.
- Authors
Abdullah, Farah Thamer; Al-Dujaily, Saad S.; Alkazaz, Abdul Kareem A.; Qassim, Ammar M.
- Abstract
Asthenozoospermia is the most common type of male infertility. Although, the mechanisms causing asthenozoospermia are complex, recent studies have indicated an importantrole of cation channel of sperm (CATSPER), gene down-regulation or gene abnormality in the etiology of asthenozoospermia in infertile patients. The present was aimed to assess gene expression and Single Nucleotide Polymorphism (rs1893316) of CATSPER 1 gene in asthenozoospermic infertile Iraqi patients. Following semen analysis, 125 men with asthenozoospermia and 40 healthy fertile control were enrolled in the present study. The patients semen samples were collected from Infertility Laboratory of Biotechnology Research Center, Al- Nahrain University and Kamal Al-Samarie IVF Hospital through the period from November 2016 to December 2017. The determinations of the SNP (rs1893316) and gene expression of CATSPER were carried out by reverse transcription quantitative polymerase chain reaction (RT-qPCR) of semen samples. The results revealed that the difference in the mean of threshold cycle (DCt) of CATSPER mRNA shown a significant (P<0.05) increase in asthenozoospermic patients compared to healthy fertile men. In terms of a relative expression, the 2–DDCt means elicited two important findings, first, the gene expression of CATSPER was down-regulated in asthenozoospermic patients. Second, nosignificant (P>0.05) variation within the subgroups of patients distributed according to asthenozoospermic condition was found. While the lowest folding was observed in oligoasthenozoospermic group when compared to other groups of infertile patients. With respect to SNPs, allele and genotype frequencies of (rs1893316) shown a significant variations (p<0.05) between asthenozoospermic men and healthy fertile men. It is concluded that the current study observed a significant reduction in the level of CATSPER 1 gene expression in asthenozoospermic patients. Moreover, the SNP of this gene may affect on asthenozoospermic Iraqi men complaining from idiopathic infertility.
- Subjects
IRAQ; SINGLE nucleotide polymorphisms; GENE expression; HUMAN fertility; REVERSE transcriptase polymerase chain reaction; BIOTECHNOLOGY research; DISEASE prevalence
- Publication
Biochemical & Cellular Archives, 2019, Vol 19, p2061
- ISSN
0972-5075
- Publication type
Article
- DOI
10.35124/bca.2019.19.S1.2061