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- Title
BIOCOMPATIBILITY INVESTIGATION AND IN VIVO EVALUATION OF KETOPROFEN ENTRAPPED IN LIPID VESICLES.
- Authors
Tarţău, Liliana; Lupuşoru, Cătălina Elena; Bîndar, Daniela; Melnig, V.
- Abstract
Nanoparticle drug carriers consists of solid biodegradable particles in size ranging from 10 to 1000 nm in which the active principle is dissolved, entrapped or encapsulated, and to which the active principle is absorbed or attached. Controlled release dosage forms cover a wide range of prolonged action formulations, which provide continuous release of their active ingredients at a predetermined rate and time. Among the drug carriers, vesicles are selfassembled colloidal particles used to encapsulate different substances within their interior, with broad usage in controlled drug release. The objective of this study consists in biocompatibility and in vivo evaluation of ketoprofen release from lipid vesicles coated in chitosan. The vesicles were prepared using an original method and thereafter, dialyzed for 10 hours, to remove their acidity. The ketoprofen vesicles were characterized for size and zetapotential by Malvern Zetasizer Nano ZS ZEN-3500. In vivo experiments were carried out on white Swiss mice (20-25 g), divided into 4 groups of 7 animals each treated orally (using an eso-gastric device) as follows: Group I (control): distilled water 0.1ml/10g body weight, Group II (Chit-lipid ves): Chitosan lipid vesicles 0.1ml/10g body weight; Group III (KET): ketoprofen 15 mg/kbw, Group IV (KET ves): ketoprofen vesicles 15 mg/kbw. The biocompatibility properties of ketoprofen vesicles were studied by assessing the effects on the blood, the serum biochemical and immune parameters. Ketoprofen release was evaluated using HPLC method, by determination of the substance blood levels in different time moments after oral administration in mice. Experimental protocol was implemented according to recommendations of the University Committee for Research. Data were statistically analyzed with SPSS software for Windows, version 17.0 and ANOVA method. We developed new carrier formulations that entrapped ketoprofen in lipid vesicles (mean size 491nm) with a moderate stability (mean Zeta potential +20.3mV). Laboratory analysis showed no significant differences of blood count, immune and biochemical parameters, between mice treated with entrapped or non entrapped ketoprofen thus indicates of good in vivo biocompatibility. The blood release kinetics study shows that ketoprofen loaded vesicles are capable of releasing the drug in a slow sustained manner. The biocompatibility studies suggest that ketoprofen lipid vesicles may be suitable for in vivo use as a drug delivery system. The use of soft matter vesicles as carriers for ketoprofen presented the advantage of drug sustained in vivo release comparing with non entrapped substance.
- Subjects
SYNAPTIC vesicles; BIOCOMPATIBILITY; NANOPARTICLES; DRUG carriers; DOSAGE forms of drugs; PARTICLES; CHITOSAN; LABORATORY mice
- Publication
Annals of the Romanian Society for Cell Biology, 2010, Vol 15, Issue 2, p347
- ISSN
2067-3019
- Publication type
Article