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- Title
Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species.
- Authors
Prado, Isabelle Gonçalves de Oliveira; da Silva, Giarlã Cunha; Crispim, Josicelli Souza; Vidigal, Pedro Marcus Pereira; Nascimento, Moysés; Santana, Mateus Ferreira; Bazzolli, Denise Mara Soares
- Abstract
Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia. Currently, there are 18 different serotypes; the serotype 8 is the most widely distributed in the United States, Canada, United Kingdom, and southeastern Brazil. In this study, genomes of seven A. pleuropneumoniae serotype 8 clinical isolates were compared to the other genomes of twelve serotypes. The analyses of serotype 8 genomes resulted in a set of 2352 protein-coding sequences. Of these sequences, 76.6% are present in all serotypes, 18.5% are shared with some serotypes, and 4.9% were differential. This differential portion was characterized as a series of hypothetical and regulatory protein sequences: mobile element sequence. Synteny analysis demonstrated possible events of gene recombination and acquisition by horizontal gene transfer (HGT) in this species. A total of 30 sequences related to prophages were identified in the genomes. These sequences represented 0.3 to 3.5% of the genome of the strains analyzed, and 16 of them contained complete prophages. Similarity analysis between complete prophage sequences evidenced a possible HGT with species belonging to the family Pasteurellaceae. Thus, mobile genetic elements, such as prophages, are important components of the differential portion of the A. pleuropneumoniae genome and demonstrate a central role in the evolution of the species. This study represents the first study done to understand the genome of A. pleuropneumoniae serotype 8.
- Subjects
ACTINOBACILLUS pleuropneumoniae; MOBILE genetic elements; HORIZONTAL gene transfer; COMPARATIVE genomics; SPECIES; AMINO acid sequence; GENOMES
- Publication
International Journal of Genomics, 2020, p1
- ISSN
2314-436X
- Publication type
Article
- DOI
10.1155/2020/9354204