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- Title
Expression, purification and crystallization of a family 55 β-1,3-glucanase from Chaetomium thermophilum.
- Authors
Papageorgiou, Anastassios C.; Li, Duochuan
- Abstract
A β-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P21, with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 Å, β = 93.1° and one molecule in the asymmetric unit.
- Subjects
GLUCANASES; CHAETOMIUM; CRYSTALLIZATION; PICHIA pastoris; GENE expression
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2015, Vol 71, Issue 6, p680
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X15006366