We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Reproducibility analysis of bioimpedance-based self-developed live cell assays.
- Authors
Vizvari, Zoltan; Gyorfi, Nina; Maczko, Gergo; Varga, Reka; Jakabfi-Csepregi, Rita; Sari, Zoltan; Furedi, Andras; Bajtai, Eszter; Vajda, Flora; Tadic, Vladimir; Odry, Peter; Karadi, Zoltan; Toth, Attila
- Abstract
Bioimpedance spectrum (BIS) measurements have a great future in in vitro experiments, meeting all the requirements for non-destructive and label-free methods. Nevertheless, a real basic research can provide the necessary milestones to achieve the success of the method. In this paper a self-developed technology-based approach for in vitro assays is proposed. Authors invented a special graphene-based measuring plate in order to assess the high sensitivity and reproducibility of introduced technique. The design of the self-produced BIS plates maximizes the detection capacity of qualitative changes in cell culture and it is robust against physical effects and artifacts. The plates do not influence the viability and proliferation, however the results are robust, stable and reproducible regardless of when and where the experiments are carried out. In this study, physiological saline concentrations, two cancer and stem cell lines were utilized. All the results were statistically tested and confirmed. The findings of the assays show, that the introduced BIS technology is appropriate to be used in vitro experiments with high efficacy. The experimental results demonstrate high correlation values across the replicates, and the model parameters suggested that the characteristic differences among the various cell lines can be detected using appropriate hypothesis tests.
- Subjects
CANCER stem cells; CELL lines; APPROPRIATE technology; CELL culture
- Publication
Scientific Reports, 2024, Vol 14, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-024-67061-2