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- Title
GADD34 gene restores virulence in viral vector used in experimental stroke study.
- Authors
McCabe, Christopher; White, Fiona; Brown, S. Moira; Macrae, I. Mhairi
- Abstract
GADD34 is expressed in the ischaemic brain and reverses protein synthesis shutdown. Consequently, GADD34 could have neuroprotective potential in stroke. BHK medium, a replication-deficient HSV viral vector (HSV1716) with no insert or containing full-length GADD34, the N terminal or a conserved portion of the gene, was injected into mouse brain before stroke. Infarct size was 1.0±0.26, 1.19±0.36, 1.5±0.36, 1.3±0.36, and 1.1±0.28 mm3, respectively. The increase in infarct size with full-length GADD34 was statistically significant (P<0.05). Immunohistochemistry confirmed viral protein expression. Tissue culture studies revealed GADD34 gene restored virulence in HSV1716, suggesting that HSV virulence, rather than increased GADD34, exacerbated ischaemic damage.Journal of Cerebral Blood Flow & Metabolism (2008) 28, 747–751; doi:10.1038/sj.jcbfm.9600565; published online 10 October 2007
- Subjects
CEREBROVASCULAR disease; ISCHEMIA; TISSUE culture; CULTURES (Biology); HEMODYNAMICS
- Publication
Journal of Cerebral Blood Flow & Metabolism, 2008, Vol 28, Issue 4, p747
- ISSN
0271-678X
- Publication type
Article
- DOI
10.1038/sj.jcbfm.9600565