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- Title
Green tea catechins evoke a phasic contraction in rat aorta via H<sub>2</sub> O<sub>2</sub> -mediated multiple-signalling pathways.
- Authors
Shen, Jian-Zhong; Zheng, Xiu-Feng; Wei, Er-Qing; Kwan, Chiu-Yin
- Abstract
Summary 1. The contractile effects of tea polyphenols (TP) and its four principle catechins, namely (–)-epicatechin (EC), (–)-epicatechin-3-gallate (ECG), (–)-epigallocatechin (EGC) and (–)-epigallocatechin-3-gallate (EGCG), on rat aorta contractility were investigated using the isometric tension recording technique. 2. At concentrations of 5–100 mg/L, TP evoked phasic contraction of rat aorta in a concentration-dependent but endothelium-independent manner. Of the four catechins tested, EGCG and EGC (3–300 µmol/L), but not EC and ECG, mimicked the contractile response to TP, suggesting that the epigallol moiety in the B ring may be associated with the contractile effect. 3. Contractions in response to EGCG and EGC were not affected by several endogenous vasoconstrictor receptor antagonists, but could be abolished by 10 µmol/L BAPTA-AM, a membrane-permeable Ca2+ chelator, or attenuated by removal of extracellular Ca2+ , suggesting the involvement of both intracellular and extracellular Ca2+ in evoking the contraction. 4. Pretreatment with non-selective Ca2+ channel antagonists mefenamic acid (10 µmol/L), tetrandrine (30 µmol/L) and SKF 96365 (30 µmol/L), but not nifedipine (1 µmol/L), the selective inhibitor of voltage-dependent Ca2+ channels, inhibited the contractile responses to EGC and EGCG, indicating the involvement of Ca2+ influx via non-voltage dependent Ca2+ channels. 5. Several intracellular Ca2+ channel modulators, including procaine (5 mmol/L), dantrolene (30 µmol/L) and 2-amino ethoxydiphenyl borate (50 µmol/L; an inositol 1,4,5-trisphosphate receptor inhibitor), also inhibited EGCG- and EGC-induced contractions, thus suggesting a role of intracellular Ca2+ release in these contractions. 6. Both EGCG- and EGC-induced contractions were depressed, to different degrees, by...
- Subjects
GREEN tea; CATECHIN; CARDIAC contraction
- Publication
Clinical & Experimental Pharmacology & Physiology, 2003, Vol 30, Issue 1/2, p88
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1046/j.1440-1681.2003.03796.x