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- Title
Essential roles of ERK-mediated phosphorylation of vinexin in cell spreading, migration and anchorage-independent growth.
- Authors
Mizutani, K.; Ito, H.; Iwamoto, I.; Morishita, R.; Deguchi, T.; Nozawa, Y.; Asano, T.; Nagata, K.-i.
- Abstract
Vinexin is an adaptor protein supposed to play pivotal roles in various cellular events such as cell adhesion, cytoskeletal organization, signaling and gene expression. Despite the possible importance, physiological functions and regulatory mechanisms of vinexin are largely unknown. In addition, although vinexin was reported to be phosphorylated by extracellular signal-regulated kinase (ERK), physiological significance of the phosphorylation remains to be elucidated. Here we carried out characterization of endogenous vinexin and found that it was enriched at the leading edge of migrating cells and focal adhesions of spread cells. In the analyses using ERK-phosphorylated vinexin-specific antibody, the phosphorylation signal was also detected at the leading edges of migrating cells and at cell periphery of spreading cells, whereas only faint signal was observed at focal adhesions of well-spread cells. We then established LNCaP cell lines stably expressing GFP-fused vinexinβ or two mutants at Ser189 that mimic the ERK-phosphorylated or -unphosphorylated vinexinβ. Based on the analyses using the lines, the phosphorylation was likely to inhibit the cell spreading and migration. On the other hand, anchorage-independent cell growth was inhibited by unphosphorylated vinexinβ. Taken together, ERK-mediated phosphorylation of vinexinβ is strongly suggested to occur in a spatio-temporally regulated manner and play important roles in cell spreading, migration and anchorage-independent growth.Oncogene (2007) 26, 7122–7131; doi:10.1038/sj.onc.1210512; published online 7 May 2007
- Subjects
CELLULAR control mechanisms; PHOSPHORYLATION; SURGICAL complications; GENE expression; CELL communication; CELL adhesion
- Publication
Oncogene, 2007, Vol 26, Issue 50, p7122
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1210512