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- Title
Effects of Depleted Uranium on Oxidative Stress, Detoxification, and Defence Parameters of Zebrafish Danio rerio.
- Authors
Gagnaire, Beatrice; Cavalie, Isabelle; Camilleri, Virginie; Adam-Guillermin, Christelle
- Abstract
In this study, we investigated the effects of depleted uranium (DU), the by-product of nuclear enrichment of uranium, on several parameters related to oxidative stress, detoxification, and the defence system in the zebrafish Danio rerio. Several parameters were recorded: phenoloxidase-like (PO) activity, reactive oxygen species (ROS) production, and 7-ethoxyresrufin- O-deethylase (EROD) activity. Experiments were performed on adult and larvae D. rerio. Adult fish were exposed for 28 days at 20 μg U/L followed by a 27-day depuration period. Eggs of D. rerio were exposed for 4 days at 0, 20, 100, 250, 500, and 1,000 μg U/L. Results showed that DU increased ROS production both in adult and in larvae even at the low concentrations tested and even during the depuration period for adult D. rerio. DU also modified PO-like activity, both in the D. rerio adult and larvae experiments, but in a more transient manner. EROD activity was not modified by DU, but sex effects were shown. Results are discussed by way of comparison with other known effects of uranium in fish. Overall, these results show that the mechanisms of action of DU in fish tend to be similar to the ones existing for mammals. These results encourage the development and use of innate immune biomarkers to understand the effects of uranium and, more generally, radionuclides on the fish immune system.
- Subjects
DEPLETED uranium; OXIDATIVE stress; DETOXIFICATION (Alternative medicine); ZEBRA danio; REACTIVE oxygen species; FISH larvae; BIOMARKERS; PARAMETER estimation
- Publication
Archives of Environmental Contamination & Toxicology, 2013, Vol 64, Issue 1, p140
- ISSN
0090-4341
- Publication type
Article
- DOI
10.1007/s00244-012-9814-z