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- Title
<em>Sclerotinia</em> rot resistance in red clover: Identification of RAPD markers using bulked segregant analysis.
- Authors
Page, D.; Delclos, B.; Aubert, G.; Bonavent, J. F.; Mousset-Déclas, C.
- Abstract
Random amplified polymorphic DNA (RAPD) was used with the objective of identifying DNA markers linked to the sclerotinia crown and stem rot (SCSR) resistance of red clover. Bulked segregant analysis was used to detect polymorphism that should be linked to SCSR resistance. Two bulks were made by pooling previously extracted DNA. Each bulk (one resistant, and the other susceptible) consisted of eight genotypes from an F2 population obtained from a cross between a susceptible and a resistant parent. A binomial model was used to select RAPD fragments with a low probability of no linkage with SCSR resistance. Four RAPD fragments were retained as candidate markers of SCSR resistance. Three are associated with resistance and one with susceptibility.
- Subjects
SCLEROTINIA trifoliorum; RED clover; RAPD technique; DISEASE resistance of plants; GENETIC markers; DNA; PROBABILITY theory
- Publication
Plant Breeding, 1997, Vol 116, Issue 1, p73
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.1997.tb00978.x