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- Title
A rapid and efficient DNA extraction method suitable for marine macroalgae.
- Authors
Ramakrishnan, Gautham; Fathima, Anwar; Ramya, Mohandass
- Abstract
Macroalgae are a diverse group of organisms. Marine macroalgae, in particular, have numerous medicinal and industrial applications. Molecular studies of macroalgae require suitable concentrations of DNA free of contaminants. At present, numerous protocols exist for DNA extraction from macroalgae. However, they are either time consuming, expensive or work only with few species. The method described in this study is rapid and efficient and applicable to different types of marine macroalgae. This method yields an average of 3.85 µg of DNA per 50 mg of algal tissue, with an average purity of 1.88. The isolated DNA was suitable for PCR amplification of universal plastid region of macroalgae.
- Subjects
NUCLEIC acid isolation methods; ALGAE; MARINE species diversity; PLASTIDS; ORGANELLES
- Publication
3 Biotech, 2017, Vol 7, Issue 6, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-017-0992-2