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- Title
Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips.
- Authors
de Graaf, Mees N. S.; Vivas, Aisen; van der Meer, Andries D.; Mummery, Christine L.; Orlova, Valeria V.
- Abstract
Organ-on-chip (OoC) devices are increasingly used to mimic the tissue microenvironment of cells in intact organs. This includes microchannels to mimic, for example, fluidic flow through blood vessels. Present methods for controlling microfluidic flow in these systems rely on gravity, rocker systems or external pressure pumps. For many purposes, pressure pumps give the most consistent flow profiles, but they are not well-suited for high throughput as might be required for testing drug responses. Here, we describe a method which allows for multiplexing of microfluidic channels in OoC devices plus the accompanying custom software necessary to run the system. Moreover, we show the approach is also suitable for recirculation of culture medium, an essential cost consideration when expensive culture reagents are used and are not "spent" through uptake by the cells during transient unidirectional flow.
- Subjects
CELL culture; BLOOD vessels; BLOOD flow
- Publication
Micromachines, 2022, Vol 13, Issue 8, p1359
- ISSN
2072-666X
- Publication type
Article
- DOI
10.3390/mi13081359