We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
TNC Accelerates Hypoxia-Induced Cardiac Injury in a METTL3-Dependent Manner.
- Authors
Cheng, Hao; Li, Linnan; Xue, Junqiang; Ma, Jianying; Ge, Junbo
- Abstract
Cardiac fibrosis and cardiomyocyte apoptosis are reparative processes after myocardial infarction (MI), which results in cardiac remodeling and heart failure at last. Tenascin-C (TNC) consists of four distinct domains, which is a large multimodular glycoprotein of the extracellular matrix. It is also a key regulator of proliferation and apoptosis in cardiomyocytes. As a significant m6A regulator, METTL3 binds m6A sites in mRNA to control its degradation, maturation, stabilization, and translation. Whether METTL3 regulates the occurrence and development of myocardial infarction through the m6A modification of TNC mRNA deserves our study. Here, we have demonstrated that overexpression of METTL3 aggravated cardiac dysfunction and cardiac fibrosis after 4 weeks after MI. Moreover, we also demonstrated that TNC resulted in cardiac fibrosis and cardiomyocyte apoptosis after MI. Mechanistically, METTL3 led to enhanced m6A levels of TNC mRNA and promoted TNC mRNA stability. Then, we mutated one m6A site "A" to "T", and the binding ability of METTL3 was reduced. In conclusion, METTL3 is involved in cardiac fibrosis and cardiomyocyte apoptosis by increasing m6A levels of TNC mRNA and may be a promising target for the therapy of cardiac fibrosis after MI.
- Subjects
HEART injuries; HEART fibrosis; HEART diseases; HEART failure; MYOCARDIAL infarction
- Publication
Genes, 2023, Vol 14, Issue 3, p591
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes14030591