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- Title
<italic>KLK10</italic> exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study.
- Authors
El Sherbini, Mustafa A.; Mansour, Amal A.; Sallam, Maha M.; Shaban, Emtiaz A.; Shehab ElDin, Zeinab A.; El-Shalakany, Amr H.
- Abstract
Background: <italic>KLK10</italic> exon 3 hypermethylation correlated to tumor-specific lack of <italic>KLK10</italic> expression in cancer cell lines and primary tumors. In the present study we investigate the possible role of <italic>KLK10</italic> exon 3 methylation in ovarian tumor diagnosis and prognosis. Results: Qualitative methylation-specific PCR (MSP) results did not show statistically significant differences in patient group samples (normal and tumor) where all samples were positive only for the unmethylated-specific PCR except for two malignant samples that were either doubly positive (serous carcinoma) or doubly negative (Sertoli-Leydig cell tumor) for the two MSP tests. However, <italic>KLK10</italic> exon 3 unmethylated PCR product concentration (ng/μl) showed statistically significant differences in benign and malignant patient group samples; mean ± SD (n): tumor: 0.077 ± 0.035 (14) and 0.047 ± 0.021 (15), respectively, <italic>p</italic>-value = 0.011; and normal: 0.094 ± 0.039 (7) and 0.046 ± 0.027 (6), respectively, <italic>p</italic>-value = 0.031. Moreover, ROC curve analysis of <italic>KLK10</italic> exon 3 unmethylated PCR product concentration in overall patient group samples showed good diagnostic ability (AUC = 0.778; <italic>p</italic>-value = 0.002). Patient survival (living and died) showed statistically significant difference according to preoperative serum CA125 concentration (U/ml); median (n): 101.25 (10) and 1252 (5), respectively, <italic>p</italic>-value = 0.037, but not <italic>KLK10</italic> exon 3 unmethylated PCR product concentration (ng/μl) in overall malignant patient samples; mean ± SD (n): 0.042 ± 0.015 (14) and 0.055 ± 0.032 (7), <italic>p</italic>-value = 0.228. Conclusion: To the best of our knowledge, this is the first report on <italic>KLK10</italic> exon 3 unmethylated PCR product concentration as potential early epigenetic diagnostic marker in primary ovarian tumors. Taken into account the limitations in our study (small sample size and semi-quantitative PCR product analysis) further studies are strongly recommended.
- Subjects
OVARIAN cancer diagnosis; POLYMERASE chain reaction; EXONS (Genetics); METHYLATION; PILOT projects
- Publication
Journal of Ovarian Research, 2018, Vol 11, Issue 1, pN.PAG
- ISSN
1757-2215
- Publication type
Article
- DOI
10.1186/s13048-018-0407-y