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- Title
Diagnostic accuracy of VIKIA® Rota‑Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger.
- Authors
Lagare, Adamou; Moumouni, Aissatou; Kaplon, Jérôme; Langendorf, Céline; Pothier, Pierre; Grais, Rebecca F.; Issaka, Bassira; Page, Anne-Laure
- Abstract
Objective: We conducted a parallel evaluation of the diagnostic accuracy of VIKIA® Rota-Adeno, a rapid diagnostic test (RDT) and Premier™ Rotaclone® an enzyme immunoassay (EIA) using reverse transcription polymerase chain reaction (RT-PCR) as the reference standard. The study was part of a rotavirus surveillance project in Niger. Results: The sensitivity of both tests was 80.7%. After exclusion of one indeterminate result by visual reading, the specificity of the Premier™ Rotaclone® was 100% by visual or optical density readings and that of VIKIA® Rota-Adeno test was 95.5%. Inter-reader agreement was excellent for both tests (kappa = 1). Our results showed almost similar performance of the EIA and RDT when compared to RT-PCR. Hence, the VIKIA® Rota-Adeno could be a good alternative for use in peripheral health centres where laboratory capacity is limited.
- Subjects
NIGER; ROTAVIRUSES; REVERSE transcriptase polymerase chain reaction; LABORATORIES; ENZYME-linked immunosorbent assay
- Publication
BMC Research Notes, 2017, Vol 10, p1
- ISSN
1756-0500
- Publication type
Article
- DOI
10.1186/s13104-017-2832-1