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- Title
Immunological Analysis of T Cells Bearing T Cell Receptor α/β or γ/δ in Patients with Granular Lymphocyte Proliferative Disorder.
- Authors
Horiuchi, Takahiko; Yasukawa, Masaki; Yanagisawa, Kohsuke; Hato, Takaaki; Fujita, Shigeru
- Abstract
Immunological analysis of 2 cases of granular lymphocyte proliferative disorder with clonal expansion of CD3+4'8+ T cells bearing either T cell receptor α/β (patient 1) or T cell receptor γ/δ (patient 2) is reported. Significant cyto-toxic activity against K562 was observed in peripheral blood mononuclear cells (PBMC) isolated from patient 2, but not in PBMC from patient 1, although the cytotoxic activity of patient 1 could be augmented following incubation with anti-CD3 monoclonal antibody (MoAb). Northern blot analysis revealed that perforin mRNA was expressed in both patients. Proliferative response to phy-tohemagglutinin, anti-CD3 MoAb and 12-0-tetradecanoylphorbol-13-acetate was low in PBMC from both patients compared to that from healthy controls. Cytoplasmic free calcium of PBMC from the 2 patients increased similarly to that of healthy controls after treatment with anti-CD3 MoAb. Copyright © 1993 S. Karger AG, Basel
- Publication
Acta Haematologica, 1993, Vol 89, Issue 4, p174
- ISSN
0001-5792
- Publication type
Article
- DOI
10.1159/000204518