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- Title
Correlation of Polymorphism of AQP7 Gene with the Sperm Cells Quality Traits of the Polish Holstein Friesian Bulls.
- Authors
Kowalczyk, Alicja; Kowalewska-Łuczak, Inga; Czerniawska-Piątkowska, Ewa; Juan Cantalapiedra, Jesús; Lima Cerqueira, Joaquim Orlando; Pinto de Araújo, José Pedro
- Abstract
The purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls.
- Subjects
MISSENSE mutation; SPERMATOZOA analysis; SPERMATOZOA; GENETIC polymorphisms; BULLS; BIOMATERIALS; SEMEN
- Publication
Pakistan Journal of Zoology, 2023, Vol 55, Issue 1, p43
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/20210901170933