We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phospholipase C signaling is involved in porcine reproductive and respiratory syndrome virus infection in cell cultures.
- Authors
YUAN, W. F.; CHEN, Q.; GAO, X. T.; ZHENG, Z. M.; JIA, H.; ZHU, H. F.; XIN, T.; SUI, X. K.; LI, M.; HOU, S. H.; GUO, X. Y.
- Abstract
The phospholipase C (PLC) is a family of kinases that hydrolyze phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] to generate two second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), which stimulate distinct downstream signaling. Recently, it has been reported that PLC signaling is activated by multiple viruses for efficient replication and the virus-induced inflammatory response. In this study, we demonstrated that PLC-specific inhibitor U73122 strongly suppressed porcine reproductive and respiratory syndrome virus (PRRSV) productive infection in cell cultures. The inhibitor affected both viral post-binding cell entry and post-entry processes. The virus infection led to an early transient activation of PLCγ-1 at 0.5 h post-infection (hpi), and sustained event at a stage from 4 to 16 hpi in MARC-145 cells. In addition, U73122 inhibited the activation of p38 MAPK signaling stimulated by PRRSV infection, suggesting that PLC signaling may be associated with the virus infection-induced inflammatory response. Taken together, these studies suggested that PLC signaling played an important role in PRRSV infection or pathogenesis.
- Subjects
PHOSPHOLIPASE C; PORCINE reproductive &; respiratory syndrome; VIRUS diseases in swine; KINASES; CELL culture
- Publication
Acta Virologica, 2019, Vol 63, Issue 1, p117
- ISSN
0001-723X
- Publication type
Article
- DOI
10.4149/av_2019_115