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- Title
Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil.
- Authors
Coelho-Melo, Maria Verônyca; Florindo-Guedes, Maria Izabel; Rodriguez-Málaga, Sergio; Magalhães de Almeida, Lia; de Freitas Moreira, Mariana; Rodrigues de Oliveira, Tatiane
- Abstract
The shrimp Litopenaeus vannamei, one of the most important species in world aquaculture, has seriously affected by infectious myonecrosis virus (IMNV) that causes up to 70% mortalities. With the aim of improving the development of new strategies for rapid and reliable diagnosis, we isolated IMNV, from L. vannamei farmed in Brazil, through a discontinuous sucrose gradient, and sequenced cDNA fragment encoding the major capsid protein from this virus. Nucleotides sequences corresponding to the viral capsid protein was obtained by RT-PCR and confirmed by automatic sequencing. Comparison with sequences which encode the capsid protein obtained from Indonesia isolates showed a high identity.
- Subjects
CEARA (Brazil : State); WHITELEG shrimp; CRUSTACEAN virus diseases; VIRUS isolation; FISH farming; AQUACULTURE; CRUSTACEAN mortality; DISEASES
- Publication
Latin American Journal of Aquatic Research, 2014, Vol 42, Issue 3, p649
- ISSN
0718-560X
- Publication type
Article
- DOI
10.3856/vol42-issue3-fulltext-22