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- Title
Distribution and Translocation of Photoreceptor Gβγ-phosducin System in Medaka Retina.
- Authors
Yamamoto, Shintaro; Hisatomi, Osamu; Hasegawa, Akiyuki; Kobayashi, Yuko; Tokunaga, Fumio
- Abstract
Unlike other vertebrates, teleosts have rod- and cone-specific phosducins (PD-R and PD-C) in the retina. To evaluate the teleost Gβγ-PD systems, we isolated cDNAs encoding medaka Gβ1 and GβC, which selectively expressed rods and cones. Immunohistochemical studies showed that the strong reactivity of GβC but not PD-C was detected in cone outer segments. In rod outer segments (ROS), PD-R reactivity was stronger in light-adapted retina than in dark-adapted retina. Western blot analyses of fractions torn from the cryosections showed that the PD-R concentration was low in dark-adapted ROS. It is suggested that PD-R is translocated to ROS and effectively downregulates the phototransduction cascade in light-adapted rods.
- Subjects
RETINA; VERTEBRATES; PHOTORECEPTORS; VISION; IMMUNOHISTOCHEMISTRY
- Publication
Photochemistry & Photobiology, 2007, Vol 83, Issue 1, p35
- ISSN
0031-8655
- Publication type
Article
- DOI
10.1562/2006-03-03-RA-834