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- Title
Molecular monitoring of treatment efficacy in human visceral leishmaniasis.
- Authors
Roy, Sutopa; Moulik, Srija; Chaudhuri, Surya Jyati; Ghosh, Manab K; Goswami, R P; Saha, Bibhuti; Chatterjee, Mitali
- Abstract
Background Focused efforts of the visceral leishmaniasis elimination program have led to a drastic decline in cases, and the present challenge is disease monitoring, which this study aimed to assess. Methods A Leishmania kinetoplastid-targeted qPCR quantified parasite load at disease presentation, and following treatment completion (n=49); an additional 80 cases were monitored after completion of treatment. Results The parasite load at disease presentation was 13 461.00 (2560.00–37764.00)/µg gDNA, which upon completion of treatment reduced in 47 of 49 cases to 1(1–1)/µg gDNA, p<0.0001. In 80 cases that presented >2 months post-treatment, their parasite burden similarly decreased to 1(1–1)/µg gDNA except in 6 of 80 cases, which were qPCR positive. Conclusion In 129 cases of visceral leishmaniasis, qPCR by quantification of parasite burden proved effective for monitoring treatment.
- Subjects
VISCERAL leishmaniasis; TREATMENT effectiveness; VISCERAL pain; BURDEN of proof; SYMPTOMS
- Publication
Transactions of the Royal Society of Tropical Medicine & Hygiene, 2024, Vol 118, Issue 5, p343
- ISSN
0035-9203
- Publication type
Article
- DOI
10.1093/trstmh/trad103