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- Title
Effects of injectable chitosan thermosensitive hydrogel on dog bone marrow stromal cells in vitro.
- Authors
HAN Xue; LIU Hong-chen; WANG Dong-sheng; SU Fang; E. Ling-ling; WU Xia
- Abstract
PURPOSE: To investigate the biologieal effects of injectable chitosan (CS) thermosensitive hydrogel on dog bone marrow stromal cells (BMSCs) in vitro. METHODS: Dog BMSCs were obtained from dog bone marrow aspiration by density gradient centrifugation method, cultured in DMEM medium with 10% fetal bovine serum (FBS) anti identified. Chitosan thermosensitive hydrogel was prepared and the BMSCs cultured in a medium containing hydrogel were observed under light microscope. The extract liquid from hydrogel was made and its effects on cell proliferation and differentiation were evaluated. Statistical significance was assessed with SPSS 13.0 software package for one -way ANOVA. RESULTS: Dog BMSCs survived in the extract liquid and there was no significant difference. The proliferation of BMSCs in mineralization condition medium was lower than normal medium after 1,4,7 days culture and was similar to that in normal medium after 10 days. The osteogenic extract liquid improved the secretion of alkaline phosphatase (ALP) and osteocalcin (OC). CONCLUSIONS: The CS thermosensitive hydrogel possesses good biocompatibility and can be used for in vitro anti in vivo experimental studies of CS hydrogel loaded with BMSCs. Supported by National Natural Science Foundation of China (30973354).
- Subjects
CHITOSAN; COLLOIDS in medicine; THERMAL properties; MESENCHYMAL stem cells; LABORATORY dogs; CELL culture; ALKALINE phosphatase
- Publication
Shanghai Journal of Stomatology, 2011, Vol 20, Issue 2, p113
- ISSN
1006-7248
- Publication type
Article