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- Title
Celiprolol induces β<sub>3</sub>-adrenoceptors-dependent relaxation in isolated porcine coronary arteries.
- Authors
Abdelkrim, Mohammed Amine; Martignat, Lionel; Gogny, Marc; Desfontis, Jean-Claude; Noireaud, Jacques; Mallem, Mohamed Yassine
- Abstract
In porcine coronary arteries (PCAs), celiprolol, a selective β1-adrenoceptors antagonist, induces vasodilatation by an endothelium- and nitric oxide (NO)-dependent pathway. However, the mechanisms of that vascular effect have not been precisely established. β3-Adrenoceptors have been shown to be involved in the relaxation per se of various vascular beds, including coronary vessels. Thus, we evaluated ( i) the presence of β3-adrenoceptors in the PCA and ( ii) their role in celiprolol-induced vasodilatation. PCA rings were placed in organ baths and preconstricted with KCl. All experiments were performed in the presence of nadolol (a β1/β2-adrenoceptor antagonist). Cumulative concentration-response curves to SR 58611A and ICI 215001 (2 β3-adrenoceptor agonists) and to celiprolol were constructed. We also used semiquantitative reverse transcription - polymerase chain reaction, which clearly showed the presence of β3-adrenoceptor transcripts. SR 58611A, ICI 215001, and celiprolol induced concentration-dependent relaxations in PCA rings. SR 58611A-induced relaxation was almost abolished after removal of endothelium or pretreatment with L-NAME (a NO synthase inhibitor). The vasorelaxations induced by SR 58611A and celiprolol were inhibited in the presence of SR 59230A and L-748337 (2 selective β3-adrenoceptor antagonists). We showed ( i) that PCAs possess functional β3-adrenoceptors mediating endothelium- and NO-dependent relaxation, and ( ii) that celiprolol exerts a β3-adrenoceptor agonistic activity in this vascular bed.
- Subjects
ADRENERGIC receptors; CORONARY disease; VASODILATION; NITRIC oxide; NADOLOL; POLYMERASE chain reaction
- Publication
Canadian Journal of Physiology & Pharmacology, 2013, Vol 91, Issue 10, p791
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/cjpp-2013-0091