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- Title
Interleukin 12 Breaks Ultraviolet Light Induced Immunosuppression by Affecting CD8[sup +] Rather Than CD4[sup +]T Cells.
- Authors
Schwarz, Agatha; Grabbe, Stephan; Mahnke, Karsten; Riemann, Helge; Luger, Thomas A.; Wysocka, Maria; Trinchieri, Giorgio; Schwarz, Thomas
- Abstract
Recent studies showed that injection of interleukin (IL)‐12 prevents ultraviolet (UV) light mediated suppression of contact hypersensitivity and breaks UV‐induced hapten specific tolerance. UV‐mediated suppression can be adoptively transferred by injecting splenocytes from UV‐irradiated mice; however, suppression is not transferable when donor mice are treated with IL‐12 after UV‐irradiation. This study was performed to elucidate the mechanisms by which IL‐12 counteracts this immunosuppression. To characterize the cells transferring suppression, depletion studies were performed revealing that UV‐induced suppression is transferred via CD8+ T cells. To investigate whether IL‐12 counteracts UV‐induced suppression by either inhibiting the development of CD8+ suppressor T cells or inducing CD4+ effector T cells, splenocytes from mice, which were IL‐12 treated and sensitized through UV‐exposed skin, were depleted from CD4+ T cells and transferred into naïve mice that were subsequently sensitized. Whereas transfer of splenocytes from UV‐irradiated mice inhibited sensitization of recipients, no inhibition was observed after transfer of splenocytes from UV‐exposed and IL‐12 treated mice. Recipients that received CD4 depleted spleen cells from UV‐exposed and IL‐12 treated donors, were still fully sensitizable. IL‐12 also blocked transfer of UV‐induced suppression when it was injected into UV‐exposed donor animals at a time point when suppressor cells had already developed. CD4 depletion of such splenocytes did not result in a loss of the reconstitutive effect of IL‐12. This suggests that IL‐12 may break UV‐induced tolerance not by inducing CD4+ effector T cells, but rather by inhibiting or inactivating suppressor T cells belonging to the CD8 subtype.
- Subjects
INTERLEUKIN-12; ULTRAVIOLET radiation; IMMUNOSUPPRESSION
- Publication
Journal of Investigative Dermatology, 1998, Vol 110, Issue 3, p272
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1046/j.1523-1747.1998.00111.x