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- Title
Identification and Characterization of Two Isozymic Forms of Arylamine N-Acetyltransferase in Syrian Hamster Skin.
- Authors
Gaudet, Stephen J.; Slominski, Andrzej; Etminan, Mohammad; Pruski, Daniel; Paus, Ralf; Namboodiri, M. A. A.
- Abstract
Arylamine N-acetyltransferase (EC 2.3.1.5) activity was examined using skin from Syrian hamster. Two isozymes of arylamine N-acetyltransferase, designated NAT-1 and NAT-2, were detected on anion-exchange high-performance liquid chromatography analysis. Both enzyme activities had indistinguishable molecular masses (30 kDa), but differed significantly in their specificity toward the aromatic amines including serotonin, dopamine, methoxytryptamine, tryptamine, para-phenetidine, para-aminobenzoic acid, and sulphamethazine. Specifically, NAT-2 but not NAT-1 catalyzed acetylation of dopamine to N-acetyldopamine and acetylation of serotonin to form N-acetylserotonin, a direct precursor of melatonin. The two isozymes were also distinguishable based upon their sensitivity toward methotrexate inhibition (50% inhibiting dose for NAT-1 = 380 μM; NAT-2 > 2 mM). The presence of these two activities in the skin raises new questions about the physiologic role of this enzyme in general and in the skin-specific functions in particular.
- Subjects
SEROTONIN; DOPAMINE; MELATONIN; ISOENZYMES; DETOXIFICATION (Alternative medicine); HIGH performance liquid chromatography
- Publication
Journal of Investigative Dermatology, 1993, Vol 101, Issue 5, p660
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12371672