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- Title
Testing UK blood donors for exposure to human parvovirus 4 using a time-resolved fluorescence immunoassay to screen sera and Western blot to confirm reactive samples.
- Authors
Maple, Peter A.C.; Beard, Stuart; Parry, Ruth P.; Brown, Kevin E.
- Abstract
Background Human parvovirus 4 ( Par V4), a newly described member of the family Parvoviridae, like B19 V, has been found in pooled plasma preparations. The extent, and significance, of Par V4 exposure in UK blood donors remain to be determined and reliable detection of Par V4 immunoglobulin ( Ig) G, using validated methods, is needed. Study Design and Methods With Par V4 virus-like particles a Par V4 IgG time-resolved fluorescence immunoassay ( TRFIA) was developed. There is no gold standard or reference assay for measuring Par V4 IgG and the utility of the TRFIA was first examined using a panel of sera from people who inject drugs ( PWIDS)-a high-prevalence population for Par V4 infection. Western blotting was used to confirm the specificity of TRFIA-reactive sera. Two cohorts of UK blood donor sera comprising 452 sera collected in 1999 and 156 sera collected in 2009 were tested for Par V4 IgG. Additional testing for B19 V IgG, hepatitis C virus antibodies (anti- HCV), and Par V4 DNA was also undertaken. Results The rate of Par V4 IgG seroprevalence in PWIDS was 20.7% and Par V4 IgG was positively associated with the presence of anti- HCV with 68.4% Par V4 IgG-positive sera testing anti- HCV-positive versus 17.1% Par V4 IgG-negative sera. Overall seropositivity for Par V4 IgG, in 608 UK blood donors was 4.76%. The Par V4 IgG seropositivity for sera collected in 1999 was 5.08%, compared to 3.84% for sera collected in 2009. No Par V4 IgG-positive blood donor sera had detectable Par V4 DNA. Conclusion Par V4 IgG has been found in UK blood donors and this finding needs further investigation.
- Subjects
UNITED Kingdom; PARVOVIRUSES; BLOOD donors; TIME-resolved fluorometry; IMMUNOASSAY; IMMUNOGLOBULIN G; WESTERN immunoblotting
- Publication
Transfusion, 2013, Vol 53, Issue 10pt2, p2575
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/trf.12278