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- Title
The Immunodeficiency of Bone Marrow- Transplanted Patients II. CD8-Related Suppression by Patient Lymphocytes of the Response of Donor Lymphocytes to Mitogens, Antigens, and Allogeneic Cells.
- Authors
&Oempty;dum, N.; Hofmann, B.; Jacobsen, N.; Langhoff, E.; M&oempty;ller, J.; Platz, P.; Ryder, L. P.; Svejgaard, A.
- Abstract
Lymphocytes from 21 patients sampled 1–6 months after bone marrow transplantation (BMT) were tested for functional suppressor activity against marrow-donor lymphocytes in the lymphocyte transformation test. Suppression of donor responses to allogeneic (i.e. mixed lymphocyte reaction, MLR) and antigenic stimulation by irradiated (7600 rad) post-BMT cells was observed in about two-thirds of the combinations tested (N=20 and N=9). The suppression of donor MLR and antigen responses ranged between 5–52% and 10–46%, respectively. Irradiated post-BMT cells significantly suppressed donor responses to suboptimal concentrations of phytohaemagglutinin (PHA) (median suppression: 28%; P<0.05; N=7) and concanavalin A (Con A) (median suppression: 31%; P<0.05; N=6). A clearly suppressive effect of post-BMT cells was observed when the ratios of CD4-/CD8+ post-BMT cells were lower than 0.5 (P<0.01). In three experiments, the depletion of the CD8- but not of the CD4-positive subset abrogated the suppression of the donor MLR by post-BMT cells. The suppression by post-BMT cells (irradiated) of MLR and mitogen responses was comparable whether the responding cells were derived from the donor or from HLA-DR-incompatible, unrelated individuals. The proliferative capacity of post-BMT cells compared to that of donor cells was assayed in the MLR with unrelated, HLA-DR-incompatible stimulator cells. A significantly decreased proliferative capacity (median 20% of that of donor cells) was found (P<0.01; N=16). A weak inverse correlation (P<0.05; N=16) between the proliferative and the suppressive capacity of post-BMT cells in the MLR was observed. These findings indicate that the decreased proliferative capacity upon mitogen, antigen, and alloantigen stimulation observed in most patients within 1–6 months after BMT may be partly due to non-specific suppression by CD8+ cells.
- Subjects
IMMUNODEFICIENCY; BONE marrow transplantation; LYMPHOCYTE receptors; SUPPRESSOR cells; T cells; IMMUNOSUPPRESSION; IMMUNE system; LYMPHOCYTES
- Publication
Scandinavian Journal of Immunology, 1987, Vol 26, Issue 3, p247
- ISSN
0300-9475
- Publication type
Article