We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1.
- Authors
Vaz, Paola K.; Mahony, Timothy J.; Hartley, Carol A.; Fowler, Elizabeth V.; Ficorilli, Nino; Lee, Sang W.; Gilkerson, James R.; Browning, Glenn F.; Devlin, Joanne M.
- Abstract
Background: While many placental herpesvirus genomes have been fully sequenced, the complete genome of a marsupial herpesvirus has not been described. Here we present the first genome sequence of a metatherian herpesvirus, Macropodid herpesvirus 1 (MaHV-1). Results: The MaHV-1 viral genome was sequenced using an Illumina MiSeq sequencer, de novo assembly was performed and the genome was annotated. The MaHV-1 genome was 140 kbp in length and clustered phylogenetically with the primate simplexviruses, sharing 67 % nucleotide sequence identity with Human herpesviruses 1 and 2. The MaHV-1 genome contained 66 predicted open reading frames (ORFs) homologous to those in other herpesvirus genomes, but lacked homologues of UL3, UL4, UL56 and glycoprotein J. This is the first alphaherpesvirus genome that has been found to lack the UL3 and UL4 homologues. We identified six novel ORFs and confirmed their transcription by RT-PCR. Conclusions: This is the first genome sequence of a herpesvirus that infects metatherians, a taxonomically unique mammalian clade. Members of the Simplexvirus genus are remarkably conserved, so the absence of ORFs otherwise retained in eutherian and avian alphaherpesviruses contributes to our understanding of the Alphaherpesvirinae. Further study of metatherian herpesvirus genetics and pathogenesis provides a unique approach to understanding herpesvirus-mammalian interactions.
- Subjects
GENOME statistics; HERPESVIRUS diseases; HERPES simplex; MACROPODIDAE; VIRAL genomes
- Publication
BMC Genomics, 2016, Vol 17, p1
- ISSN
1471-2164
- Publication type
Article
- DOI
10.1186/s12864-016-2390-2