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- Title
Unconjugated interferon-stimulated gene 15 specifically interacts with the hepatitis C virus NS5A protein via domain I.
- Authors
Minami, Nanae; Abe, Takayuki; Deng, Lin; Matsui, Chieko; Fukuhara, Takasuke; Matsuura, Yoshiharu; Shoji, Ikuo
- Abstract
ABSTRACT Interferon-stimulated gene 15 (ISG15), a ubiquitin-like protein, is induced by type I INF. Although several groups have reported ISGylation of the HCV NS5A protein, it is still unclear whether ISGylation of NS5A has anti- or pro-viral effects in hepatitis C virus (HCV) infection. In the present study, the role of ISGylation-independent, unconjugated ISG15 in HCV infection was examined. Immunoprecipitation analyses revealed that ISG15 interacts specifically with NS5A domain I. ISG15 mutants lacking the C-terminal glycine residue that is essential for ISGylation still interacted with NS5A protein. Taken together, these results suggest that unconjugated ISG15 affects the functions of HCV NS5A through protein-protein interaction.
- Subjects
INTERFERONS; HEPATITIS C virus; PROTEIN-protein interactions; VIRAL nonstructural proteins; IMMUNOPRECIPITATION
- Publication
Microbiology & Immunology, 2017, Vol 61, Issue 7, p287
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/1348-0421.12493