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- Title
Stepwise changes in viable but nonculturable Vibrio cholerae cells.
- Authors
Imamura, Daisuke; Mizuno, Tamaki; Miyoshi, Shin‐ichi; Shinoda, Sumio
- Abstract
ABSTRACT Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.
- Subjects
VIBRIO cholerae; CELL culture; BACTERIAL growth; RESUSCITATION; ADENOCARCINOMA; FLUORESCENCE microscopy
- Publication
Microbiology & Immunology, 2015, Vol 59, Issue 5, p305
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/1348-0421.12246