We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens.
- Authors
Jiang, Liying; Lan, Xianghao; Ren, Linjiao; Jin, Zhiyuan; Shan, Xuchen; Yang, Mingzhu; Chang, Lingqian
- Abstract
To minimize and control the transmission of infectious diseases, a sensitive, accurate, rapid, and robust assay strategy for application on-site screening is critical. Here, we report single-molecule RNA capture-assisted digital RT-LAMP (SCADL) for point-of-care testing of infectious diseases. Target RNA was captured and enriched by specific capture probes and oligonucleotide probes conjugated to magnetic beads, replacing laborious RNA extraction. Droplet generation, amplification, and the recording of results are all integrated on a microfluidic chip. In assaying commercial standard samples, quantitative results precisely corresponded to the actual concentration of samples. This method provides a limit of detection of 10 copies mL−1 for the N gene within 1 h, greatly reducing the need for skilled personnel and precision instruments. The ultrasensitivity, specificity, portability, rapidity and user-friendliness make SCADL a competitive candidate for the on-site screening of infectious diseases.
- Subjects
NUCLEIC acid probes; RNA; MEDICAL screening; INFECTIOUS disease transmission; COMMUNICABLE diseases; OLIGONUCLEOTIDES
- Publication
Microsystems & Nanoengineering, 2023, Vol 9, Issue 1, p1
- ISSN
2096-1030
- Publication type
Article
- DOI
10.1038/s41378-023-00576-2