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- Title
Genetic and epigenetic X-chromosome variations in a parthenogenetic human embryonic stem cell line.
- Authors
Liu, Weiqiang; Yin, Yifei; Jiang, Yonghua; Kou, Chaohui; Luo, Yumei; Huang, Shengchang; Zheng, Yuhong; Li, Shaoying; Li, Qing; Guo, Liyuan; Gao, Shaorong; Sun, Xiaofang
- Abstract
Purpose: To assess the genetic and epigenetic status of parthenogenetic human embryonic stem cells (phESCs). Methods: Cytogenetics, X chromosome inactivation (XCI) and gene expression patterns were analyzed in one phESC line (FY-phES-018) that was derived from our laboratory. Results: FY-phES-018 cells displayed the classical characteristics of normal hESCs. These cells had a 46, XX karyotype, and no inactive X chromosomes were observed before passage 20. After being cultured long term in vitro, some cells lost one X, and the proportion of cells with only one X gradually increased. At passage 35, almost all the cells displayed a 45, XO karyotype. Interestingly, at passage 45, the recovery of the X-chromosome was observed, and XCI became detectable; the mosaic ratio of 46, XX to 45, XO was 67:33. After passage 60, most cells displayed the 46, XX karyotype again with a mosaic ratio of 97:3. Some aberrant genomic imprinting was also observed in these cells. Conclusions: The phESCs line FY-phES-018 is both genetically and epigenetically unstable; therefore, further research is needed before using these cells.
- Subjects
X chromosome; BIOLOGICAL variation; EMBRYONIC stem cells; GENE expression; KARYOTYPES; GENOMICS; FERTILIZATION in vitro
- Publication
Journal of Assisted Reproduction & Genetics, 2011, Vol 28, Issue 4, p303
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-010-9517-1