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- Title
Classy non-wovens based on animate L. gasseri-inanimate poly(vinyl alcohol): upstream application in food engineering.
- Authors
Amna, Touseef; Hassan, M.; Pandeya, Dipendra; Khil, Myung-Seob; Hwang, I.H.
- Abstract
We explored electrospinning as a feasible and practicable mode for encapsulation and stabilization of Lactobacillus gasseri. The utilized nanocomposite was prepared using sol-gel composed of animate L. gasseri and inanimate PVA. The objective was to examine the ability of electrospinning method to protect functional properties of probiotic L. gasseri. The PVA was used as an encapsulation matrix as it is biocompatible and hydrophilic in nature thus facilitate an easy revival of bacteria. The characterization of as-spun bioproduct was done by energy-dispersive X-ray spectrometer, SEM, and TEM, whereas thermal behavior was analyzed by thermogravimetry. The viability was confirmed by traditional pour plate method and fluorescence microscopy. Furthermore, to test whether the functionality of L. gasseri was affected, the encapsulated L. gasseri were fed to mouse for colonization. Our results pointed out that encapsulated bacteria were viable for months, and their metabolism was not affected by immobilization; thus, they could be used in food engineering and trade.
- Subjects
ELECTROSPINNING; LACTOBACILLUS gasseri; NANOCOMPOSITE materials; MICROENCAPSULATION; X-ray spectrometers; THERMOGRAVIMETRY; FLUORESCENCE microscopy
- Publication
Applied Microbiology & Biotechnology, 2013, Vol 97, Issue 10, p4523
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-012-4666-z