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- Title
Urinary microbiome differences between lichen sclerosus induced and non-lichen sclerosus induced urethral stricture disease.
- Authors
Jamil, Marcus L.; Perecman, Aaron; Sherman, Amanda; Sullivan, Travis; Christ, Kimberly; Hansma, Alexandra; Burks, Eric; Vanni, Alex J.
- Abstract
Objective: To describe differences in the urinary microbiome of patients with pathologically confirmed lichen sclerosus (LS) urethral stricture disease (USD) vs non-lichen sclerosus (non-LS) USD pre- and post-operatively. Methods: Patients were pre-operatively identified and prospectively followed, all underwent surgical repair and had tissue samples obtained to make a pathological diagnosis of LS. Pre- and post-operative urine samples were collected. Bacterial genomic DNA was extracted. Alpha and beta diversity measurements were calculated and compared. A zero-inflated negative binomial model was utilized to compare taxa abundances between disease status and surgery status. Results: Urine samples were obtained from both cohorts, 69 samples in total: 36 samples were obtained pre-operatively and 33 samples were obtained post-operatively. Ten patients provided both a pre-operative and post-operative urine sample. Twenty-six patients had pathological evidence of LS and 33 patients did not. There was a statistically significant difference in alpha diversity between the pre-operative urine samples of patients with non-LS USD and LS USD, (p = 0.01). There was no significant difference in alpha diversity within post-operative urine samples between patients with non-LS USD and LS USD, (p = 0.1). A significant difference was observed in Weighed UniFrac distances with respect to disease and operative status, (p = 0.001 and 0.002). Conclusions: LS USD have significant alterations in diversity and differential abundance of urine microbiota compared to non-LS USD controls. These findings could be used to guide further investigations into the role of the urinary microbiome in LS USD pathogenesis, severity of presentation, and stricture recurrence.
- Subjects
URETHRA stricture; LICHEN sclerosus et atrophicus; URETHRA diseases; URINE collection &; preservation; BACTERIAL DNA; U.S. dollar
- Publication
World Journal of Urology, 2023, Vol 41, Issue 9, p2495
- ISSN
0724-4983
- Publication type
Article
- DOI
10.1007/s00345-023-04490-0