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- Title
双水相临界萃取条件优化方法及其应用.
- Authors
曾颖; 曾淑欣; 何玉书; 林韫琦
- Abstract
The enzyme activity and extraction effect of β-glucosidase in the aqueous two-phase subphase extracts of abalone viscera, scallop viscera and oyster viscera were investigated by using“5² ×3 aqueous two-phase extraction method” to optimize the extraction conditions. The results showed that the critical system optimized by this method was 12%PEG4000/15% NaH2PO4. The β-glucosidase of subphase extracts extracted by this system showed the trend of oyster viscera>abalone viscera>scallop viscera in terms of enzyme activity, specific activity, purification ratio, and recovery.
- Subjects
VISCERA; ABALONES; SCALLOPS; OYSTERS; ENZYMES
- Publication
Food Research & Development, 2023, Vol 44, Issue 16, p100
- ISSN
1005-6521
- Publication type
Article
- DOI
10.12161/j.issn.1005-6521.2023.16.014