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- Title
Hydrogel Matrix to Support Stem Cell Survival After Brain Transplantation in Stroke.
- Authors
Jin Zhong; Chan, Albert; Morad, Leeron; Kornblum, Harley I.; Guoping Fan; Carmichael, S. Thomas
- Abstract
Stroke is a leading cause of adult disability. Stem/progenitor cell transplantation improves recovery after stroke in rodent models. These studies have 2 main limitations to clinical translation. First, most of the cells in stem/progenitor transplants die after brain transplantation. Second, intraparenchymal approaches target transplants to normal brain adjacent to the stroke, which is the site of the most extensive natural recovery in humans. Transplantation may damage this tissue. The stroke cavity provides an ideal target for transplantation because it is a compartmentalized region of necrosis, can accept a high volume transplant without tissue damage, and lies directly adjacent to the most plastic brain area in stroke. However, direct transplantation into the stroke cavity has caused massive death in the transplant. To overcome these limitations, the authors tested stem/progenitor transplants within a specific biopolymer hydrogel matrix to create a favorable environment for transplantation into the infarct cavity after stroke, and they tested this in comparison to stem cell injection without hydrogel support. A biopolymer hydrogel composed of cross-linked hyaluronan and heparin sulfate significantly promoted the survival of 2 different neural progenitor cell lines in vitro in conditions of stress and in vivo into the infarct cavity. Quantitative analysis of the transplant and surrounding tissue indicates diminished inflammatory infiltration of the graft with the hydrogel transplant. This result indicates that altering the local environment in stem cell transplantation enhances survival and diminishes cell stress. Stem cell transplantation into the infarct cavity within a pro-survival hydrogel matrix may provide a translational therapy for stroke recovery.
- Subjects
BRAIN physiology; SURGERY; CEREBROVASCULAR disease; COLLAGEN; THERAPEUTIC use of hyaluronic acid; STEM cell transplantation; ANIMAL experimentation; BIOLOGICAL models; BIOPHYSICS; BIOPOLYMERS; BRAIN; CELL differentiation; CELL physiology; CELL transplantation; CELL motility; COMPARATIVE studies; COMPUTER software; GRAFT versus host reaction; HEPARIN; HYALURONIC acid; IMMUNOHISTOCHEMISTRY; INFLAMMATION; RESEARCH methodology; MICE; MICROSCOPY; RESEARCH funding; STEM cells; PSYCHOLOGICAL stress; T-test (Statistics); DATA analysis; QUANTITATIVE research; PHARMACODYNAMICS; THERAPEUTICS
- Publication
Neurorehabilitation & Neural Repair, 2010, Vol 24, Issue 7, p636
- ISSN
1545-9683
- Publication type
Article
- DOI
10.1177/1545968310361958