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- Title
两株氢氧化细菌(zw-17, zw-35)对重金属 胁迫下白菜幼苗生长的影响.
- Authors
周晓伦; 牛李莹; 郑宁国; 王卫卫
- Abstract
Hydrogen oxidizing bacteria belonging to the plant growth promoting rhizobacteria, so the mechanism for the growth of exploration has become a focus attention at home and abroad, in which there are few researches on plant growth promoting of hydrogen oxidizing bacteria under heavy metals stress. In order to study whether the hydrogen oxidizing bacteria has growth promotion and protection to the Pak choi seedlings under heavy metal stress. We used two strains hydroxide bacteria and cabbage seedlings as the research materials. The strain species identification was determined by conventional and molecular biology method. Using NJ adjacent method to construct phylogenetic tree, ACC deaminase activity was estimated by 2, 4-dinitrobenzene hydrazine method, the ability of IAA was determined by Salkowski reagent method, the plant root elongation promoting activity of the isolated hydrogen oxidizing bacteria was determined by the modified Belimov method. Wilkins method was used to test the resistance of Pak choi seedlings tolerance index. The results showed that two hydrogen oxidizing bacteria respectively belong to Pseudomonas sp. zw-17 and Sphingobacterium sp. zw-35. The ACC deaminase activity was (17. 87±0. 58), (22. 26±0. 85)μmol a-KB?mg-1?h-1. The ability of IAA was (13. 21±0. 78) to (5. 79±5. 79)mg?L-1. Compared to the control group, inoculating hydroxide bacteria was benefit to the growth of the Pak choi seedlings root. Compared to the control group, tolerance index of Pak choi seedlings had significant increase when inoculated with zw-17, zw-35. The results of tolerance index and physiological indicators for Pak choi seedlings, obtained for chromium and lead for both hydro-gen oxidizing bacteria strains, showed that the development of plants inoculated with the hydrogen-oxidizing bacteria Pseudomonas sp. zw-17 strain favored the radical growth of Pak choi seedlings exposed to Pb and Pseudomonas sp. zw-3 5 strain in seedlings exposed to Cr; both strains keep the safe development of this species in the presence of the heavy metals at higher concentrations. This study would provide a theoretical basis for hydrogen oxidizing bacteria promoting and protecting plants, and enriched hydroxide bacteria species classification.
- Publication
Guihaia, 2015, Vol 35, Issue 5, p673
- ISSN
1000-3142
- Publication type
Article
- DOI
10.11931/guihaia.gxzw201407048