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- Title
A Study of the Expression of ANGPT1, ANGPT2 in Mouse Model of Acute Myeloid Leukemia by Using Recombinant ANGPT2 and Monoclonal Antibody against ANGPT2.
- Authors
YANG Hong; WANG Wei-ya; ZHOU Ze-ping; LIU Lin; ZHANG You; GAO Chun - lin
- Abstract
Objective To investigate the expressions of ANGPT1/2 proteins in the tumor tissue of mouse model of acute myeloid leukemia by using recombinant ANGPT2 and monoclonal antibody against ANGPT2. Methods HL-60 cells were transplanted with NOD/SCID mouse through abdominal injection into construct mouse model of acute myeloid leukemia. Then the mice were injected with recombinant ANGPT2 and monoclonal antibody against ANGPT2. The expressions of ANGPT1 and ANGPT2 proteins in the tumor tissues of mouse model were detected by Western Blot. Results In recombinant ANGPT2, there were no significant differences in the expressions of ANGPT1/2 between the control group and experimental group. In anti-ANGPT2 group, there was no significant difference in the expression of ANGPT2 between the control group and experimental group, while the expression of ANGPT1 was significantly lower than the control group. Conclusion In the future, VEGF should be blocked if we want achieve the good results using the ANGPT2 monoclonal antibody.
- Subjects
ACUTE myeloid leukemia treatment; RECOMBINANT antibodies; THERAPEUTIC use of monoclonal antibodies; LABORATORY mice; CONTROL groups
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2014, Vol 35, Issue 11, p78
- ISSN
2095-610X
- Publication type
Article