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- Title
MicroRNA 182 is a Novel Negative Regulator of Adipogenesis by Targeting CCAAT/Enhancer-Binding Protein α.
- Authors
Dong, Meijuan; Ye, Yuqing; Chen, Zhinan; Xiao, Ting; Liu, Wei; Hu, Fang
- Abstract
<bold>Objective: </bold>Recent studies have shown that microRNAs (miRNAs/miRs) play key roles in adipogenesis. This study aimed to investigate the role and underlying mechanism of miR-182 in adipogenesis.<bold>Methods: </bold>This study used the 3T3-L1 cell line and human visceral adipose tissue (VAT)-derived adipocytes to determine the role of miR-182 in adipogenesis. Adipose tissues from mice with high-fat diet-induced obesity, ob/ob mice, or human individuals with obesity were used to determine the association of miR-182 levels with obesity. A luciferase reporter assay was used to determine the target of miR-182.<bold>Results: </bold>The expression level of miR-182 was greatly downregulated during white adipogenesis and markedly lower in the VAT of mice and humans with obesity. Ectopic expression of miR-182 in 3T3-L1 cells and human adipocytes suppressed the formation of lipid droplets and the expression of adipogenic genes. The luciferase reporter assay showed that miR-182 targeted the 3'-untranslated sequence of CCAAT/enhancer-binding protein α (C/EBPα) directly. In addition, glucocorticoids negatively regulated miR-182 expression, which, in turn, suppressed the glucocorticoid-induced expression of C/EBPα.<bold>Conclusions: </bold>Taken together, our studies identified miR-182 as a novel negative regulator of adipogenesis and a potential therapeutic target for obesity.
- Subjects
ADIPOGENESIS; ADIPOSE tissues; MICRORNA; OBESITY; AMINO acid sequence; RNA metabolism; CELL metabolism; PROTEIN metabolism; CELL differentiation; BIOCHEMISTRY; RESEARCH; ANIMAL experimentation; RESEARCH methodology; CELL physiology; MEDICAL cooperation; EVALUATION research; PHENOMENOLOGY; COMPARATIVE studies; FAT cells; GENETIC techniques; MICE
- Publication
Obesity (19307381), 2020, Vol 28, Issue 8, p1467
- ISSN
1930-7381
- Publication type
journal article
- DOI
10.1002/oby.22863