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- Title
A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies.
- Authors
Thippu Jayaprakash, Kamalram; Mostafa, Denis; Hussein, Mohammad; Shaffer, Richard; Michael, Agnieszka; Ajaz, Mazhar; Nisbet, Andrew
- Abstract
Featured Application: Preliminary in vitro screening of radiation–drug combinations within a clinical set-up. We designed and developed a multiwell tissue culture plate irradiation setup, and intensity modulated radiotherapy plans were generated for 96-, 24-, and 6-well tissue culture plates. We demonstrated concordance between planned and measured/imaged radiation dose profiles using radiochromic film, a 2D ion chamber array, and an electronic portal-imaging device. Cell viability, clonogenic potential, and γ-H2AX foci analyses showed no significant differences between intensity-modulated radiotherapy and open-field, homogeneous irradiations. This novel platform may help to expedite radiobiology experiments within a clinical environment and may be used for wide-ranging ex vivo radiobiology applications.
- Subjects
RADIOBIOLOGY; INTENSITY modulated radiotherapy; IONIZATION chambers; LINEAR accelerators; PHOTONS; RADIATION doses
- Publication
Applied Sciences (2076-3417), 2022, Vol 12, Issue 3, p1456
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app12031456