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- Title
Silencing of AP-4 inhibits proliferation, induces cell cycle arrest and promotes apoptosis in human lung cancer cells.
- Authors
XUANYU HU; WEI GUO; SHANSHAN CHEN; YIZHUO XU; PING LI; HUAQI WANG; HEYING CHU; JUAN LI; YUWEN DU; XIAONAN CHEN; GUOJUN ZHANG; GUOQIANG ZHAO
- Abstract
Activating enhancer-binding protein (AP)-4 is a member of the basic helix-loop-helix transcription factors, and is involved in tumor biology. However, the role of AP-4 in human lung cancer remains to be fully elucidated. In the present study, the expression of AP-4 in human lung cancer tissues and cells was investigated by reverse transcription-quantitative polymerase chain reaction, and it was observed that the level of AP-4 was increased in tumor tissues and cells compared with their normal counterparts. AP-4 expression was knocked down by transfection with a specific small interfering RNA (siRNA) in lung cancer cells, and this indicated that siRNA-mediated silencing of AP-4 inhibited cell proliferation, arrested the cell cycle at the G0/G1 phase and induced apoptosis by modulating the expression of p21 and cyclin D1. The results of the present study suggest that AP-4 may be an oncoprotein that has a significant role in lung cancer, and that siRNA-mediated silencing of AP-4 may have therapeutic potential as a strategy for the treatment of lung cancer.
- Subjects
LUNG cancer &; genetics; GENE silencing; LUNG cancer treatment; THERAPEUTIC use of RNA interference; CANCER cell proliferation; APOPTOSIS; REVERSE transcriptase polymerase chain reaction
- Publication
Oncology Letters, 2016, Vol 11, Issue 6, p3735
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2016.4451