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- Title
A unique basal pattern of p53 expression in ulcerative colitis is associated with mutation in the p53 gene.
- Authors
Noffsinger, A E; Belli, J M; Miller, M A; Fenoglio-Preiser, C M
- Abstract
A unique basal pattern of p53 expression in ulcerative colitis is associated with mutation in the p53gene Aims: The p53 protein is implicated in the control of cell proliferation, differentiation, and death. As part of a study characterizing p53 alterations in colonic mucosa of patients with ulcerative colitis, we identified a unique pattern of basal p53 immunoreactivity. Methods and results: Tissue samples (n=180) from 42 ulcerative colitis patients were evaluated for p53 alterations by immunohistochemistry, loss of heterozygosity analysis, polymerase chain reaction-single-strand conformation polymorphism and direct sequencing. In addition, the expression of the p53- associated proteins p21waf1/cip1 and MDM2 was evaluated immunohistochemically. Three basic patterns of p53 immunoreactivity were observed: (i) isolated immunoreactive cells in the crypt bases; (ii) strongly positive cells confined to the basal half of the glands; and (iii) diffusely staining cells. The basal staining pattern was observed in both non-neoplastic tissues and in some areas of dysplasia, and was associated with normal expression of p21waf1/cip1 in all cases, and with p53 mutation in seven of 11 cases. Conclusions: The basal pattern of p53 expression is associated with mutation in the p53 gene, and appears to be an early change in a subgroup of ulcerative colitis patients. The significance of this pattern of immunoreactivity and the mechanism by which it develops are discussed.
- Subjects
P53 antioncogene; ULCERATIVE colitis
- Publication
Histopathology, 2001, Vol 39, Issue 5, p482
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1046/j.1365-2559.2001.01274.x