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- Title
The Cap Snatching of Segmented Negative Sense RNA Viruses as a Tool to Map the Transcription Start Sites of Heterologous Co-infecting Viruses.
- Authors
Wenzhong Lin; Ping Qiu; Jing Jin; Shunmin Liu; Ul Islam, Saif; Jinguang Yang; Jie Zhang; Kormelink, Richard; Zhenguo Du; Zujian Wu
- Abstract
Identification of the transcription start sites (TSSs) of a virus is of great importance to understand and dissect the mechanism of viral genome transcription but this often requires costly and laborious experiments. Many segmented negative-sense RNA viruses (sNSVs) cleave capped leader sequences from a large variety of mRNAs and use these cleaved leaders as primers for transcription in a conserved process called cap snatching. The recent developments in high-throughput sequencing have made it possible to determine most, if not all, of the capped RNAs snatched by a sNSV. Here, we show that rice stripe tenuivirus (RSV), a plant-infecting sNSV, co-infects Nicotiana benthamiana with two different begomoviruses and snatches capped leader sequences from their mRNAs. By determining the 5' termini of a single RSV mRNA with high-throughput sequencing, the 5' ends of almost all themRNAs of the co-infecting begomoviruses could be identified and mapped on their genomes. The findings in this study provide support for the using of the cap snatching of sNSVs as a tool to map viral TSSs.
- Subjects
RNA viruses; VIRAL genomes; MESSENGER RNA
- Publication
Frontiers in Microbiology, 2017, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2017.02519