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- Title
Molecular diagnosis of trichodorid species from Portugal.
- Authors
Duarte, I. M.; Almeida, M. T. M.; Duarte, M. M.; Brown, D. J. F.; Neilson, R.
- Abstract
A PCR-RFLP assay was developed for the identification of trichodorid nematodes belonging to Nanidorus, Paratrichodorus and Trichodorus genera . Using the variability of the 18S SSU rDNA gene, this method provides a new molecular diagnosis tool which allows identification within mixed samples of trichodorid and non-trichodorid species, differentiation of juveniles, and represents an alternative to the difficult and time consuming phenotyping of similar species. Based on the alignment of previously obtained 18S rDNA nucleotide sequences of trichodorids from Portugal, a pair of selective primers was designed in conserved regions to allow the amplification of a variable region located at the 3′ end of the gene in all known Portuguese species. The 615 bp PCR product showed nucleotide variability enabling the generation of restriction fragment patterns which were consistent among populations of the same species but allowed discrimination of trichodorids at the species level. The proposed protocol was tested and proved effective with 12 trichodorid species from Portugal ( N. minor, P. allius, P. anemones, P. divergens, P. hispanus, P. pachydermus, P. porosus, T. beirensis, T. lusitanicus, T. primitivus and two other Trichodorus species, A and B) and six non-indigenous trichodorid populations ( N. minor, P. allius, P. anemones, P. pachydermus, P. porosus and T. primitivus).
- Subjects
PORTUGAL; MOLECULAR diagnosis; HOST plants of nematodes; PLANT parasites; TRICHODORUS; PESTICIDES
- Publication
Plant Pathology, 2011, Vol 60, Issue 3, p586
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/j.1365-3059.2010.02401.x