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- Title
Evidence of asexual recombination in <em>Rhynchosporium secalis</em>.
- Authors
Newman, Pamela L.; Owen, H.
- Abstract
Three single-spore isolates of Rhynchosporium secalis that differed in their α-esterase and β-glucosidase isozyme patterns were inoculated as two mixtures, each of two isolates, on to seedlings of the susceptible barley cultivar Maris Mink. Approximately 100 single-spore isolates were taken from mature lesions produced by each mixture. These were subjected to polyacrylamide gel electrophoresis and the gels were stained for α-esterase and β-glucosidase. Parental types only were produced by one of the isolate mixtures. However, one of the 10 lesions examined for the second mixture produced nine isolates with a novel combination of isozymes, indicating that some form of asexual recombination had occurred. The use of isozymes as a natural marker system for the detection in vivo of asexual recombination in pathogenic fungi is discussed.
- Subjects
RHYNCHOSPORIUM secalis; BARLEY varieties; POLYACRYLAMIDE gel electrophoresis; ASEXUAL reproduction; PATHOGENIC fungi; BIOMARKERS
- Publication
Plant Pathology, 1985, Vol 34, Issue 3, p338
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/j.1365-3059.1985.tb01370.x